PRESERVATION OF STOCK CULTURES OF BACTERIA BY FREEZING AND DRYING

Abstract

Attention is called to the fact that bacteria may be preserved for a long time by desiccation in the frozen state. It has been shown that it is necessary to maintain the frozen condition until desiccation is complete; if the fluid melts before the moisture is completely removed, the organisms are killed, probably because of the concentration of the salts upon the surface of the bacteria. By the simple expedient of immersing the tubes of organisms in glycerol contained in a desiccator and subsequently keeping the whole apparatus in a salt-ice mixture until drying is complete, the organisms are easily maintained in the frozen state, and dry properly. Bacteria preserved in this manner retain their cultural, biochemical, and immunological characters for prolonged periods.