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. 1923 Apr 30;37(5):647-51.
doi: 10.1084/jem.37.5.647.

STABLE SUSPENSIONS OF AUTOAGGLUTINABLE BACTERIA

P H De Kruif  1 , J H Northrop
Affiliations

STABLE SUSPENSIONS OF AUTOAGGLUTINABLE BACTERIA

P H De Kruif et al. J Exp Med. .

Abstract

Autoagglutinating cultures of Type G, bacillus of rabbit septicemia, can be rendered stable by suspension in glycocoll-acetate-phosphate buffer mixtures pH 7.5 and 7.1. Distilled water suspensions can also be employed if the normal and immune serum under test is diluted with buffers of this pH instead of with 0.85 per cent NaCl. Five intensely autoagglutinable strains of Streptococcus haemolyticus have been stabilized by washing and by suspending in 0.001 N NaOH. Specific agglutination of these stable suspensions by rabbit antistreptococcus serum could be demonstrated by diluting the serum with M/320 NaCl.

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References

    1. J Exp Med. 1919 Sep 1;30(3):179-213 - PubMed
    1. J Exp Med. 1922 Aug 31;36(3):309-16 - PubMed