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. 1931 Oct 31;54(5):681-99.
doi: 10.1084/jem.54.5.681.

IN VITRO TRANSFORMATION OF PNEUMOCOCCAL TYPES : I. A TECHNIQUE FOR INDUCING TRANSFORMATION OF PNEUMOCOCCAL TYPES IN VITRO

M H Dawson  1 , R H Sia
Affiliations

IN VITRO TRANSFORMATION OF PNEUMOCOCCAL TYPES : I. A TECHNIQUE FOR INDUCING TRANSFORMATION OF PNEUMOCOCCAL TYPES IN VITRO

M H Dawson et al. J Exp Med. .

Abstract

1. Type-specific S pneumococci may be transformed from one specific S type into other specific S types entirely by in vitro methods. 2. R forms of pneumococci, derived from S forms of one specific type, may be transformed into S forms of other specific types by the following in vitro procedure:-the growth of small inocula of R forms in media containing vaccines prepared from heterologous S cultures. 3. Transformation of type may be effected in this procedure by the use of small quantities of S vaccine,-quantities representing the bacteria from as little as 0.1 cc. of the original culture. 4. Transformation of type, as induced by this procedure, is most readily effected by employing anti-R serum in the culture medium. Transformation of type may be effected, however, in media which do not contain anti-R antibodies. 5. Previous findings on the thermal characteristics of the property of S vaccines responsible for transformation of type have been confirmed and extended.

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References

    1. J Exp Med. 1926 Jan 1;43(1):107-17 - PubMed
    1. J Exp Med. 1925 Aug 31;42(3):367-76 - PubMed
    1. J Exp Med. 1930 Jan 1;51(1):99-122 - PubMed
    1. J Exp Med. 1928 Mar 31;47(4):577-91 - PubMed
    1. J Exp Med. 1930 Jan 1;51(1):123-47 - PubMed