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. 1932 Mar 31;55(4):555-61.
doi: 10.1084/jem.55.4.555.

QUANTITATIVE STUDIES ON THE PRECIPITIN REACTION : THE DETERMINATION OF SMALL AMOUNTS OF A SPECIFIC POLYSACCHARIDE

M Heidelberger  1 , F E Kendall
Affiliations

QUANTITATIVE STUDIES ON THE PRECIPITIN REACTION : THE DETERMINATION OF SMALL AMOUNTS OF A SPECIFIC POLYSACCHARIDE

M Heidelberger et al. J Exp Med. .

Abstract

A method, based on the precipitin reaction, is given for the micro determination of the specific polysaccharide of Type III pneumococcus. As little as 0.01 mg. of S III can be determined. The method should be applicable to any specific polysaccharide upon standardization of an homologous antibody solution or antiserum in the region of excess antibody.

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References

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    1. J Exp Med. 1929 Nov 30;50(6):809-23 - PubMed
    1. J Exp Med. 1930 Sep 30;52(4):477-83 - PubMed