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. 1937 Feb 28;65(3):393-414.
doi: 10.1084/jem.65.3.393.

THE MOLECULAR WEIGHT OF ANTIBODIES

M Heidelberger  1 , K O Pedersen
Affiliations

THE MOLECULAR WEIGHT OF ANTIBODIES

M Heidelberger et al. J Exp Med. .

Abstract

1. Highly purified rabbit Type III pneumococcus anticarbohydrate proved to be homogeneous in the ultracentrifuge and its sedimentation constant, 7.0.10(-13), did not differ from that of the principal component of normal rabbit globulin or of immune rabbit globulin containing up to 50 per cent of anti-egg albumin. The molecular weight of antibody in the rabbit is therefore probably very close to that of the principal normal globulin component, namely, 150,000. 2. Highly purified horse Type I pneumococcus anticarbohydrate, on the other hand, was only homogeneous in the ultracentrifuge when prepared from sera stored without preservative. Its sedimentation constant, 18.4.10(-13), coincided with that of the principal globulin component in most of the Felton solutions and purified antibody solutions studied. The molecular weight of pneumococcus anticarbohydrate in the horse is probably three to four times that of the principal normal globulin component. 3. The significance of the differences between pneumococcus anticarbohydrate formed in the rabbit and in the horse is discussed. 4. Results are given of ultracentrifuge studies on the molecular species in solutions of egg albumin-anti-egg albumin specific precipitates dissolved in excess egg albumin. The implications of the results are discussed.

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