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. 1939 Sep 30;70(4):379-85.
doi: 10.1084/jem.70.4.379.

ESTIMATION OF THE PURITY OF PREPARATIONS OF ELEMENTARY BODIES OF VACCINIA

J E Smadel  1 , T M Rivers, E G Pickels
Affiliations

ESTIMATION OF THE PURITY OF PREPARATIONS OF ELEMENTARY BODIES OF VACCINIA

J E Smadel et al. J Exp Med. .

Abstract

A method of estimating the purity of preparations of elementary bodies of vaccinia is described. It depends on the comparison of the number of infective units of virus in a given material with the number of elementary bodies. The latter figure is estimated from the dry weight of the preparation by means of a calculated value for the weight of a single dehydrated elementary body. Values for the ratio of infective units of vaccine virus to elementary bodies varied between 1 : 2.4 and 1 : 9.2 in seven consecutive experiments; the average was 1:4.2. These ratios indicate a high degree of purity of the preparation. Moreover, they indicate that a relatively high percentage of the elementary bodies in the preparations was infective.

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References

    1. J Exp Med. 1935 Aug 31;62(3):349-52 - PubMed
    1. J Exp Med. 1936 Aug 31;64(3):439-52 - PubMed
    1. J Exp Med. 1938 Apr 30;67(5):725-38 - PubMed
    1. J Exp Med. 1936 Sep 30;64(4):503-28 - PubMed
    1. J Exp Med. 1935 Jun 30;62(1):65-72 - PubMed