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. 1991 Jan;173(1):404-6.
doi: 10.1128/jb.173.1.404-406.1991.

Chromogenic method for rapid isolation of recA-like mutants of gram-negative bacteria

J Barbe  1 , A R Fernandez de Henestrosa, S Calero, I Gibert
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Chromogenic method for rapid isolation of recA-like mutants of gram-negative bacteria

J Barbe et al. J Bacteriol. 1991 Jan.

Abstract

We have devised a rapid and widely applicable color test for detecting recA-like mutants of gram-negative bacteria. The technique depends on decreased expression of an Escherichia coli recA-lacZ fusion in recA mutants and uses a broad-host-range plasmid to transfer the fusion gene into new species. We describe the isolation of a recA-like mutant of Pseudomonas syringae by this technique.

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