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. 1943 Dec 1;78(6):465-76.
doi: 10.1084/jem.78.6.465.

STUDIES ON THE ANTIGENIC COMPOSITION OF GROUP A HEMOLYTIC STREPTOCOCCI : I. EFFECTS OF PROTEOLYTIC ENZYMES ON STREPTOCOCCAL CELLS

R C Lancefield  1
Affiliations

STUDIES ON THE ANTIGENIC COMPOSITION OF GROUP A HEMOLYTIC STREPTOCOCCI : I. EFFECTS OF PROTEOLYTIC ENZYMES ON STREPTOCOCCAL CELLS

R C Lancefield. J Exp Med. .

Abstract

1. Proteolytic enzymes destroy the type-specific M antigen of group A hemolytic streptococci not only when the M substance is contained in cell-free extracts but also when it is a component of the living cell. 2. The injection of enzyme-treated cultures into rabbits fails to induce the formation of M antibodies, but does result in the production of T antibodies and, therefore, provides a method of preparing anti-T sera free from M antibodies. 3. Exposure to these enzymes does not kill the bacteria. Virulence and the ability to form M substance are restored on subculture and animal inoculation. 4. The study of the effect of proteolytic enzymes on group A hemolytic streptococci provides new techniques for analyzing the antigenic composition of these microorganisms.

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