Development of behavior in the litter huddle in rat pups: within- and between-litter differences

Abstract

Early postnatal growth in mammals can be considerably influenced by litter size and often differs among littermates in relation to birth mass. In a study of Long Evans laboratory rats we asked whether within- and between-litter differences in body mass and growth are related to behavioral development during early postnatal life. For this, we analyzed the amount of general motor activity and the display of directed, seemingly goal-oriented interactions within the litter huddle in previsual pups. During the study period from postnatal days 2 to 11, we found significant changes in pup behavior, showing a nonlinear, quadratic shape. General motor activity and, more specifically, the display of behaviors apparently directed to reaching central positions in the litter huddle increased during the first postnatal days and then decreased again. However, pups from small litters that grow more rapidly than pups from large litters, showed a faster increase in both behaviors, whereas the young from large litters reached a higher maximum. We also found striking within-litter differences in the amount of directed behavior performed by light and heavy pups, with higher levels in the former group, most probably because light pups that have a less favorable body mass-to-volume ratio and more often occupy peripheral positions in the litter huddle, make a greater effort to reach thermally favorable central positions. In conclusion, our study shows there to be consistent between-litter as well as within-litter differences in behavioral patterns during early life. These differences might have important implications for an individual's long-term behavioral and physiological performance.