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Review
. 2009 Dec;13(5-6):687-96.
doi: 10.1016/j.cbpa.2009.09.030. Epub 2009 Oct 29.

Non-natural nucleic acids for synthetic biology

Daniel H Appella  1
Affiliations
Review

Non-natural nucleic acids for synthetic biology

Daniel H Appella. Curr Opin Chem Biol. 2009 Dec.

Abstract

Genetic manipulation is an important facet of synthetic biology but can be complicated by undesired nuclease degradation. Incorporating non-natural nucleic acids into a gene could convey resistance to nucleases and promote expression. The compatibility of non-natural nucleosides with polymerases is reviewed with a focus on results from the past two years. Details are provided about how the different systems could be useful in synthetic biology.

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References

    1. Luisi PL. Chemical aspects of synthetic biology. Chem Biodivers. 2007;4:603–621. - PubMed
    1. Sismour AM, Benner SA. Synthetic biology. Expert Opin Biol Ther. 2005;5:1409–1414. - PubMed
    1. Benner SA, Sismour AM. Synthetic biology. Nat Rev Genet. 2005;6:533–543. - PMC - PubMed
    1. Potthast T. Paradigm shifts versus fashion shifts?Systems and synthetic biology as new epistemic entities in understanding and making ‘life’. EMBO Rep. 2009;10 1:S42–45. - PMC - PubMed
    1. Morange M. A new revolution? The place of systems biology and synthetic biology in the history of biology. EMBO Rep. 2009;10 1:S50–53. - PMC - PubMed

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