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Review
. 2009 Dec;13(5-6):687-96.
doi: 10.1016/j.cbpa.2009.09.030. Epub 2009 Oct 29.

Non-natural nucleic acids for synthetic biology

Daniel H Appella  1
Affiliations
Review

Non-natural nucleic acids for synthetic biology

Daniel H Appella. Curr Opin Chem Biol. 2009 Dec.

Abstract

Genetic manipulation is an important facet of synthetic biology but can be complicated by undesired nuclease degradation. Incorporating non-natural nucleic acids into a gene could convey resistance to nucleases and promote expression. The compatibility of non-natural nucleosides with polymerases is reviewed with a focus on results from the past two years. Details are provided about how the different systems could be useful in synthetic biology.

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