Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1977 Sep;74(9):3715-9.
doi: 10.1073/pnas.74.9.3715.

Reconstitution of catecholamine-sensitive adenylate cyclase activity: interactions of solubilized components with receptor-replete membranes

E M Ross, A G Gilman

Reconstitution of catecholamine-sensitive adenylate cyclase activity: interactions of solubilized components with receptor-replete membranes

E M Ross et al. Proc Natl Acad Sci U S A. 1977 Sep.

Abstract

Membranes of mouse L cells that contain adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] but lack beta-adrenergic receptors have been solubilized with Lubrol 12A9. Addition of such adenylate cyclase-containing extracts to beta-adrenergic receptor-replete membranes from adenylate cyclase-deficient S49 lymphoma cells results in the production of a catecholamine-sensitive adenylate cyclase system. The effects of beta-adrenergic agonists and antagonists on the reconstituted system reproduce those that are characteristic of the wild-type S49 lymphoma cell. The uncoupled variant of the S49lymphoma contains adenylate cyclase, but donor extracts from this clone fail to reconstitute the hormone-sensitive enzyme activity when added to adenylate cyclase-deficient membranes. Thus, the uncoupled and adenylate cyclase-deficient variants of the S49 cell are not complementary.

PubMed Disclaimer

References

    1. J Biol Chem. 1951 Nov;193(1):265-75 - PubMed
    1. Biochim Biophys Acta. 1976 Dec 8;452(2):538-51 - PubMed
    1. Adv Cyclic Nucleotide Res. 1977;8:1-83 - PubMed
    1. J Biol Chem. 1977 Jan 25;252(2):799-802 - PubMed
    1. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4410-4 - PubMed

Publication types

MeSH terms

LinkOut - more resources