Purification of simian virus 40 tumor antigen from a line of simian virus 40-transformed human cells
- PMID: 198802
- PMCID: PMC431712
- DOI: 10.1073/pnas.74.9.3745
Purification of simian virus 40 tumor antigen from a line of simian virus 40-transformed human cells
Abstract
Simian virus 40 tumor antigen can be isolated in a highly purified state from the nuclei ofSV80 cells, a continuous line of simian virus 40-transformed human fibroblasts. A five-step purification method was used. Its apparent molecular weight (in sodium dodecyl sulfate/polyacrylamide gels) is approximately 90,000-94,000. It contains a detectable amino-terminal residue.
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