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. 1977 Sep;74(9):3745-9.
doi: 10.1073/pnas.74.9.3745.

Purification of simian virus 40 tumor antigen from a line of simian virus 40-transformed human cells

Purification of simian virus 40 tumor antigen from a line of simian virus 40-transformed human cells

D G Tenen et al. Proc Natl Acad Sci U S A. 1977 Sep.

Abstract

Simian virus 40 tumor antigen can be isolated in a highly purified state from the nuclei ofSV80 cells, a continuous line of simian virus 40-transformed human fibroblasts. A five-step purification method was used. Its apparent molecular weight (in sodium dodecyl sulfate/polyacrylamide gels) is approximately 90,000-94,000. It contains a detectable amino-terminal residue.

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