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Review
. 2009:463:239-58.
doi: 10.1016/S0076-6879(09)63016-0.

Tagging for protein expression

Arun Malhotra  1
Affiliations
Review

Tagging for protein expression

Arun Malhotra. Methods Enzymol. 2009.

Abstract

Tags are frequently used in the expression of recombinant proteins to improve solubility and for affinity purification. A large number of tags have been developed for protein production and researchers face a profusion of choices when designing expression constructs. Here, we survey common affinity and solubility tags, and offer some guidance on their selection and use.

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