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Review
. 2009:463:349-71.
doi: 10.1016/S0076-6879(09)63022-6.

Ion-exchange chromatography

Alois Jungbauer  1 , Rainer Hahn
Affiliations
Review

Ion-exchange chromatography

Alois Jungbauer et al. Methods Enzymol. 2009.

Abstract

Ion-exchange chromatography is the most popular chromatographic method for separation of proteins. It is a versatile and generic tool and is suited for discovery of proteins, high-resolution purification, and industrial production of proteins. Separation conditions are within physiological range of salt and pH and in the most cases a native protein can be obtained. In this chapter, the guidance will be provided for binding and elution conditions and selection of stationary phases.

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