Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2009 Nov-Dec;26(11-12):583-9.
doi: 10.1007/s10815-009-9355-1. Epub 2009 Nov 6.

Cytogenetic analysis of human embryos and embryonic stem cells derived from monopronuclear zygotes

Hongqing Liao  1 , Shuoping Zhang, Dehua Cheng, Qi Ouyang, Ge Lin, Yifan Gu, Changfu Lu, Fei Gong, Guangxiu Lu
Affiliations

Cytogenetic analysis of human embryos and embryonic stem cells derived from monopronuclear zygotes

Hongqing Liao et al. J Assist Reprod Genet. 2009 Nov-Dec.

Abstract

Purpose: By comparing the chromosomal constitution among the arrested cleavage-stage embryos, blastocysts and human embryonic stem cells (hESCs) which are all derived from monopronuclear (1PN) zygotes, it is aimed to determine whether chromosomally normal embryos can be reliably selected by blastocyst culture.

Methods: After 1PN zygotes are sequentially cultured for 5 days, the blastocysts and arrested cleavage-stage embryos were analyzed by fluorescence in situ hybridization (FISH) with probes for chromosomes 18, X and Y; G-banding analysis was adopted to analyze the karyotype of 1PN hESCs.

Results: The diploid rate of blastocysts was 74.6%, which was significantly (P < 0.001) higher than that of arrested cleavage-stage embryos (31.6%), and the diploid rate of hESCs was 97.0%, which was significantly (P < 0.01) higher than that of blastocysts; the haploid embryos were excluded by blastocyst culture; nevertheless, there still existed such chromosomal abnormalities as mosaic and monosomic in blastocysts and trisomy in hESCs.

Conclusions: Blastocyst culture is an effective method to select against chromosomal abnormalities, especially the haploids in 1PN embryos; however, development to the blastocyst stage is not a reliable marker for mosaicism or aneuploidy.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
a FISH analysis of an arrested cleavage-stage 1PN embryo showing haploid: one 18 chromosome signal (blue) and one X chromosome signal (green) in each nuclear; b FISH analysis of a 1PN blastocyst showing diploid: two 18 chromosome signals (blue), one X chromosome signal (green) and one Y chromosome signal (red) in each nuclear; c G-banding analysis of chHES-22-P2 cells: 46, XY; d G-banding analysis of chHES-22-P75 cells: 46, XY
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. {'text': '', 'ref_index': 1, 'ids': [{'type': 'DOI', 'value': '10.1007/s10815-004-8758-2', 'is_inner': False, 'url': 'https://doi.org/10.1007/s10815-004-8758-2'}, {'type': 'PMC', 'value': 'PMC3455616', 'is_inner': False, 'url': 'https://pmc.ncbi.nlm.nih.gov/articles/PMC3455616/'}, {'type': 'PubMed', 'value': '15704517', 'is_inner': True, 'url': 'https://pubmed.ncbi.nlm.nih.gov/15704517/'}]}
    2. Dasig D, Lyon J, Behr B, Milki AA. Monozygotic twin birth after the transfer of a cleavage stage embryo resulting from a single pronucleated oocyte. Journal of Assisted Reproduction and Genetics. 2004;21:427–9. - PMC - PubMed
    1. {'text': '', 'ref_index': 1, 'ids': [{'type': 'PubMed', 'value': '8473423', 'is_inner': True, 'url': 'https://pubmed.ncbi.nlm.nih.gov/8473423/'}]}
    2. Staessen C, Janssenswillen C, Devroey P, Van Steirteghem AC. Cytogenetic and morphological observations of single pronucleated human oocytes after in-vitro fertilization. Hum Reprod. 1993;8:221–3. - PubMed
    1. {'text': '', 'ref_index': 1, 'ids': [{'type': 'PubMed', 'value': '7836530', 'is_inner': True, 'url': 'https://pubmed.ncbi.nlm.nih.gov/7836530/'}]}
    2. Nagy ZP, Liu J, Joris H. Time-course of oocyte activation, pronucleus formation and cleavage in human oocytes fertilized by intracytoplasmic sperm injection. Hum Reprod. 1994;9:1743–8. - PubMed
    1. {'text': '', 'ref_index': 1, 'ids': [{'type': 'DOI', 'value': '10.1093/humrep/12.3.532', 'is_inner': False, 'url': 'https://doi.org/10.1093/humrep/12.3.532'}, {'type': 'PubMed', 'value': '9130755', 'is_inner': True, 'url': 'https://pubmed.ncbi.nlm.nih.gov/9130755/'}]}
    2. Payne D, Flaherty SP, Barry MF, Matthews CD. Preliminary observations on polar body extrusion and pronuclear formation in human oocytes using time-lapse video cinematography. Hum Reprod. 1997;12:532–41. - PubMed
    1. {'text': '', 'ref_index': 1, 'ids': [{'type': 'DOI', 'value': '10.1095/biolreprod52.3.653', 'is_inner': False, 'url': 'https://doi.org/10.1095/biolreprod52.3.653'}, {'type': 'PubMed', 'value': '7756458', 'is_inner': True, 'url': 'https://pubmed.ncbi.nlm.nih.gov/7756458/'}]}
    2. Levron J, Munne S, Willadsen S, Rosenwaks Z, Cohen J. Male and female genomes associated in a single pronucleus in human zygotes. Biol Reprod. 1995;52:653–7. - PubMed

Publication types