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Review
. 2010;50(1):190-200.
doi: 10.1016/j.advenzreg.2009.10.010. Epub 2009 Nov 6.

The guanine nucleotide exchange factor (GEF) Ect2 is an oncogene in human cancer

Alan P Fields  1 , Verline Justilien
Affiliations
Review

The guanine nucleotide exchange factor (GEF) Ect2 is an oncogene in human cancer

Alan P Fields et al. Adv Enzyme Regul. 2010.
No abstract available

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Figures

Figure 1
Figure 1. Schematic diagram of the domain structure of the Ect2 protein
N, Amino-terminal region; XRCC1, X-ray repair complementing defective repair in Chinese hamster cells 1 domain; Cyclin B6, cyclin B6-like domain; BRCT, BRAC1 C-terminal domain; S, small central region; NLS, nuclear localization sequence; DH, Dbl homology domain; PH, pleckstrin homology domain; C, Carboxyl-terminal region.
Figure 2
Figure 2. Ect2 is mislocalized to the cytoplasm of primary non-small cell lung cancer tumor cells
Immunohistochemical staining of Ect2 in normal human lung epithelium (left panel) and a primary lung adenocarcinoma (right panel). Ect2 localizes predominantly to the nucleus of normal lung epithelial cells, but is localized to both the nucleus and cytoplasm of lung cancer cells.
Figure 3
Figure 3. PKCι and Par6α regulate the cytoplasmic localization of Ect2 in NSCLC cells
Immunofluorescence staining of H1703 NSCLC cells expressing Non-target (NT) RNAi, PKCι RNAi or Par6α RNAi for Ect2 (red) and DAPI (blue). Ect2 is localized to both the nucleus and cytoplasm (arrows) of NT cells, but redistributes to the nucleus of PKCι RNAi and Par6α RNAi cells.
Figure 4
Figure 4. Schematic diagram of the PKCι-Par6α-Ect2 oncogenic signaling axis
Cytoplasmic Ect2 binds to the PKCι-Par6α complex and activates a Rac1/Pak1/Mek 1,2/Erk 1,2 signaling axis that drives transformed growth and invasion of NSCLC cells.
See this image and copyright information in PMC

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