Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2010 Jun;22(6):597-603.
doi: 10.1002/chir.20798.

Direct enantioselective HPLC monitoring of lipase-catalyzed kinetic resolution of flurbiprofen

Ashraf Ghanem  1
Affiliations

Direct enantioselective HPLC monitoring of lipase-catalyzed kinetic resolution of flurbiprofen

Ashraf Ghanem. Chirality. 2010 Jun.

Abstract

The solvent versatility of Chiralpak IB, a 3,5-dimethylphenylcarbamate derivative of cellulose-based chiral stationary phase, is demonstrated in the direct enantioselective HPLC monitoring of lipase-catalyzed kinetic resolution of flurbiprofen in nonstandard HPLC organic solvents. Nonstandard HPLC organic solvents were used as the reaction media for the lipase-catalysis and in mean time as diluent to dissolve the "difficult to dissolve" enzyme substrate (the acid) and as eluent for the simultaneous enantioselective HPLC baseline separation of both substrate and product in one run without any further derivatization.

PubMed Disclaimer

LinkOut - more resources