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. 2009 Nov-Dec;26(11-12):613-9.
doi: 10.1007/s10815-009-9362-2. Epub 2009 Nov 11.

Vitrification of bovine oocytes: implications of follicular size and sire on the rates of embryonic development

Vahida M Anchamparuthy  1 , Arindam Dhali, Whitney M Lott, Ronald E Pearson, Francis C Gwazdauskas
Affiliations

Vitrification of bovine oocytes: implications of follicular size and sire on the rates of embryonic development

Vahida M Anchamparuthy et al. J Assist Reprod Genet. 2009 Nov-Dec.

Abstract

Purpose: The objectives were to test how the source of oocytes and semen impacted vitrification of large numbers of bovine oocytes and subsequent IVF and early embryo development to test procedures that may assist with assisted reproductive technologies in humans.

Methods: Bovine oocytes were vitrified from follicles of different diameters, small (< or =4 mm) and medium (4 to 10 mm), using nylon mesh. Oocytes were exposed to the cryoprotectant composed of 40% (v/v) ethylene glycol, 18% (w/v) Ficoll-70, and 0.3 M sucrose in three stepwise dilutions. Thawing was conducted with a series of 0.5, 0.25 and 0.125 M sucrose dilutions in 20% fetal bovine serum.

Results: The cleavage (39.1% vs. 58.5%) and blastocyst rates (5.1% vs. 22.9%) were significantly lower for the vitrified oocytes. Follicle size had a significant impact on the development of embryos. Sires had significant effects on embryonic developmental rates.

Conclusions: We conclude that differences in development exist due to follicle source and sire used for IVF after vitrification.

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Figures

Fig. 1
Fig. 1
Morphology of oocytes at 15 h of maturation. a and b without vitrification, c and d vitrified (presence of intact cumulus cells). Left (100 ×), and right (40 ×) magnification. Both vitrified and non-vitrified oocytes had homogenous ooplasm, intact membranes and zona pellucida
Fig. 2
Fig. 2
Sire influence on the in vitro development of embryos, developmental rate ± SE (%)
See this image and copyright information in PMC

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