Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Randomized Controlled Trial
. 2009 Nov-Dec;26(11-12):575-81.
doi: 10.1007/s10815-009-9357-z. Epub 2009 Nov 11.

An auto-controlled prospective comparison of two embryos culture media (G III series versus ISM) for IVF and ICSI treatments

Christophe Sifer  1 , Deborah Handelsman, Emilie Grange, Raphael Porcher, Christophe Poncelet, Brigitte Martin-Pont, Brigitte Benzacken, Jean-Philippe Wolf
Affiliations
Randomized Controlled Trial

An auto-controlled prospective comparison of two embryos culture media (G III series versus ISM) for IVF and ICSI treatments

Christophe Sifer et al. J Assist Reprod Genet. 2009 Nov-Dec.

Abstract

Purpose: To compare the effects of 2 different media on embryo morphology and development at days 2/3.

Method: Six hundred seventy-six attempts from 512 couples were included in this prospective auto-controlled study. Sibling oocytes of all couples undergoing an IVF (n = 286) or ICSI (n = 390) attempt were randomly assigned to either GIII series (Vitrolife) or ISM (Medicult) media. Primary end points were fertilization and embryo morphology rates.

Results: Fertilization rates in GIII series and ISM (IVF: 59.9 vs 62.0% and ICSI: 65.7 vs 66.8%) respectively were not different. GIII series showed an increase, compared to ISM, of early cleavage rate, (IVF: 25.8 vs 16.2% (p = 0.005); ICSI: 40.8 vs 25.5% (p < 0.0001), and good embryo morphology rate at day 2 [IVF: 64.6 vs 57.3% (p = 0.01); ICSI: 74.2 vs 69.4 (p = 0.03)] and at day 3 [IVF: 57.5 vs 49.0% (p = 0.02); ICSI: 67.2 vs 61.6% (p = 0.01)].

Conclusions: Embryo morphology at days 2/3 was significantly enhanced when the embryos were cultured in GIII series.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
The consort flowchart of oocytes through the trial
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. {'text': '', 'ref_index': 1, 'ids': [{'type': 'DOI', 'value': '10.1093/humupd/1.2.91', 'is_inner': False, 'url': 'https://doi.org/10.1093/humupd/1.2.91'}, {'type': 'PubMed', 'value': '15726768', 'is_inner': True, 'url': 'https://pubmed.ncbi.nlm.nih.gov/15726768/'}]}
    2. Bavister BD. Culture of preimplantation embryos: facts and artifacts. Hum Reprod Update. 1995;1:91–148. - PubMed
    1. {'text': '', 'ref_index': 1, 'ids': [{'type': 'DOI', 'value': '10.1093/humrep/14.7.1842', 'is_inner': False, 'url': 'https://doi.org/10.1093/humrep/14.7.1842'}, {'type': 'PubMed', 'value': '10402402', 'is_inner': True, 'url': 'https://pubmed.ncbi.nlm.nih.gov/10402402/'}]}
    2. Karamalegos C, Bolton VN. A prospective comparison of 'in house' and commercially prepared Earle's balanced salt solution in human in-vitro fertilization. Hum Reprod. 1999;14:1842–6. - PubMed
    1. {'text': '', 'ref_index': 1, 'ids': [{'type': 'PubMed', 'value': '3902512', 'is_inner': True, 'url': 'https://pubmed.ncbi.nlm.nih.gov/3902512/'}]}
    2. Quinn P, Kerin JF, Warnes GM. Improved pregnancy rate in human in vitro fertilization with the use of a medium based on the composition of human tubal fluid. Fertil Steril. 1985;44:493–8. - PubMed
    1. {'text': '', 'ref_index': 1, 'ids': [{'type': 'DOI', 'value': '10.1023/B:JARG.0000043702.35570.56', 'is_inner': False, 'url': 'https://doi.org/10.1023/b:jarg.0000043702.35570.56'}, {'type': 'PMC', 'value': 'PMC3455441', 'is_inner': False, 'url': 'https://pmc.ncbi.nlm.nih.gov/articles/PMC3455441/'}, {'type': 'PubMed', 'value': '15568329', 'is_inner': True, 'url': 'https://pubmed.ncbi.nlm.nih.gov/15568329/'}]}
    2. Ben-Yosef D, Amit A, Azem F, Schwartz T, Cohen T, Mei-Raz N, et al. Prospective randomized comparison of two embryo culture systems: P1 medium by Irvine Scientific and the Cook IVF Medium. J Assist Reprod Genet. 2004;21:291–5. - PMC - PubMed
    1. {'text': '', 'ref_index': 1, 'ids': [{'type': 'PubMed', 'value': '8283458', 'is_inner': True, 'url': 'https://pubmed.ncbi.nlm.nih.gov/8283458/'}]}
    2. Conaghan J, Handyside AH, Winston RM, Leese HJ. Effects of pyruvate and glucose on the development of human preimplantation embryos in vitro. J Reprod Fertil. 1993;99:87–95. - PubMed

Publication types

LinkOut - more resources