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. 2009 Nov 14;15(42):5287-94.
doi: 10.3748/wjg.15.5287.

Mucosal bacterial microflora and mucus layer thickness in adolescents with inflammatory bowel disease

Affiliations

Mucosal bacterial microflora and mucus layer thickness in adolescents with inflammatory bowel disease

Krzysztof Fyderek et al. World J Gastroenterol. .

Abstract

Aim: To assess the mucosa-associated bacterial microflora and mucus layer in adolescents with inflammatory bowel disease (IBD).

Methods: Sixty-one adolescents (mean age 15 years, SD+/-4.13) were included in the study. Intestinal biopsies from inflamed and non-inflamed mucosa of IBD patients and from controls with functional abdominal pain were cultured under aerobic and anaerobic conditions. The number of microbes belonging to the same group was calculated per weight of collected tissue. The mucus thickness in frozen samples was measured under a fluorescent microscope.

Results: The ratios of different bacterial groups in inflamed and non-inflamed mucosa of IBD patients and controls were specific for particular diseases. Streptococcus spp. were predominant in the inflamed mucosa of Crohn's disease (CD) patients (80% of all bacteria), and Lactobacillus spp. were predominant in ulcerative colitis patients (90%). The differences were statistically significant (P=0.01-0.001). Lower number of bifidobacteria was observed in the whole IBD group. A relation was also found between clinical and endoscopic severity and decreased numbers of Lactobacillus and, to a lesser extent, of Streptococcus in biopsies from CD patients. The mucus layer in the inflamed sites was significantly thinner as compared to controls (P=0.0033) and to non-inflamed areas in IBD patients (P=0.031).

Conclusion: The significantly thinner mucosa of IBD patients showed a predominance of some aerobes specific for particular diseases, their numbers decreased in relation to higher clinical and endoscopic activity of the disease.

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Figures

Figure 1
Figure 1
Relationships between endoscopic activity and total numbers of bacteria cultured from biopsies of UC patients and the control group. Likelihood ratio = 9.535, P = 0.0490. UC: Ulcerative colitis.
Figure 2
Figure 2
Relationships between disease activity and numbers of Lactobacillus group of bacteria cultured from biopsies of CD patients and the control group. Likelihood ratio = 13.209, P = 0.0398. CD: Crohn’s disease.
Figure 3
Figure 3
The average thickness of the mucus layer in biopsy samples obtained from inflamed areas in CD (H = 8.0, P = 0.0047) and UC (H = 6.76, P = 0.0093) patients and controls. The horizontal line represents median value with 25% and 75% quartile.
Figure 4
Figure 4
The average thickness of the mucus layer in biopsy samples obtained from non-inflamed areas in CD (H = 0.1481, P = 0.7003) and UC (H = 0.987, P = 0.3137) patients and controls. The horizontal line represents median value with 25% and 75% quartile.

References

    1. Hanauer SB. Inflammatory bowel disease: epidemiology, pathogenesis, and therapeutic opportunities. Inflamm Bowel Dis. 2006;12 Suppl 1:S3–S9. - PubMed
    1. Sokol H, Seksik P, Rigottier-Gois L, Lay C, Lepage P, Podglajen I, Marteau P, Doré J. Specificities of the fecal microbiota in inflammatory bowel disease. Inflamm Bowel Dis. 2006;12:106–111. - PubMed
    1. Seksik P, Sokol H, Lepage P, Vasquez N, Manichanh C, Mangin I, Pochart P, Doré J, Marteau P. Review article: the role of bacteria in onset and perpetuation of inflammatory bowel disease. Aliment Pharmacol Ther. 2006;24 Suppl 3:11–18. - PubMed
    1. Giaffer MH, Holdsworth CD, Duerden BI. The assessment of faecal flora in patients with inflammatory bowel disease by a simplified bacteriological technique. J Med Microbiol. 1991;35:238–243. - PubMed
    1. Sellon RK, Tonkonogy S, Schultz M, Dieleman LA, Grenther W, Balish E, Rennick DM, Sartor RB. Resident enteric bacteria are necessary for development of spontaneous colitis and immune system activation in interleukin-10-deficient mice. Infect Immun. 1998;66:5224–5231. - PMC - PubMed

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