doi: 10.1016/0304-4165(91)90208-x.
Purification of histone H10 and its subfractions under non-denaturing conditions
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- PMID: 1991142
- DOI: 10.1016/0304-4165(91)90208-x
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Purification of histone H10 and its subfractions under non-denaturing conditions
Biochim Biophys Acta.
.
Abstract
A quick and convenient method for a non-denaturing purification of histone H10 and its subfractions from different mammalian sources is presented. It is based on cation-exchange chromatography on a CM-Sephadex C-25 column developed by a linear gradient of NaCl. The procedure allows a satisfactory fractionation of H10 subfractions in a form suitable for further studies in assays requiring the native state of the protein.
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