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. 1991 Jan 23;1073(1):230-2.
doi: 10.1016/0304-4165(91)90208-x.

Purification of histone H10 and its subfractions under non-denaturing conditions

T Banchev  1 , L Srebreva, J Zlatanova
Affiliations

Purification of histone H10 and its subfractions under non-denaturing conditions

T Banchev et al. Biochim Biophys Acta. .

Abstract

A quick and convenient method for a non-denaturing purification of histone H10 and its subfractions from different mammalian sources is presented. It is based on cation-exchange chromatography on a CM-Sephadex C-25 column developed by a linear gradient of NaCl. The procedure allows a satisfactory fractionation of H10 subfractions in a form suitable for further studies in assays requiring the native state of the protein.

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