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Review
. 2010;50(1):349-62.
doi: 10.1016/j.advenzreg.2009.10.024. Epub 2009 Nov 13.

Sphingosine 1-phosphate lyase, a key regulator of sphingosine 1-phosphate signaling and function

Montserrat Serra  1 , Julie D Saba
Affiliations
Review

Sphingosine 1-phosphate lyase, a key regulator of sphingosine 1-phosphate signaling and function

Montserrat Serra et al. Adv Enzyme Regul. 2010.
No abstract available

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Figures

Figure 1
Figure 1
Scheme of sphingolipid metabolism. Long-chain bases are formed by de novo synthesis or by degradation of higher order sphingolipids. S1P and ceramide have opposite effects in the regulation of cell metabolism.
Figure 2
Figure 2
Sequence alignment of SPL homologs. ClustalW alignment of SPL amino acid sequences of Homo sapiens (NP 003892), Mus musculus (NP 033189), Drosophila melanogaster (NP 652032), Caenorhabditis elegans (NP 499913), Saccharomyces cerevisiae (XP 751779), Dictyostelium discoideum (XP 639378), Leishmania major (CAJ06500) and Arabidopsis thaliana (AAG51494). Identical residues are indicated in black, and similar residues are indicated in gray. The underlined regions indicate the ER luminal domain (red), the transmembrane spanning domain (green) and the predicted pyridoxal phosphate binding (purple).
Figure 3
Figure 3
Structure of fluorescent substrates for SPL activity assays. BODIPY substrates have a different emission wavelength depending on the concentration of the label. Coumarinic substrate only shows fluorescence when it is not linked to the S1P backbone.
Figure 4
Figure 4
Chemical structure of known SPL inhibitors. Other than THI, all the inhibitors shown have a chemical structure mimicking S1P.
See this image and copyright information in PMC

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