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. 2009 Nov:Chapter 17:17.5.1-17.5.17.
doi: 10.1002/0471142735.im1705s87.

Preparation of stable single-chain trimers engineered with peptide, beta2 microglobulin, and MHC heavy chain

Ted Hansen  1 , Y Y Lawrence Yu  1 , Daved H Fremont  1
Affiliations

Preparation of stable single-chain trimers engineered with peptide, beta2 microglobulin, and MHC heavy chain

Ted Hansen et al. Curr Protoc Immunol. 2009 Nov.

Abstract

This unit describes a method for constructing a class I MHC molecule with a bound peptide as a single polypeptide chain, termed SCT, for single chain trimer. The component organization of the SCT appears to be widely applicable to different mouse or human MHC class I isotypes bound by different antigenic peptides. The enhanced peptide occupancy afforded by the SCT format makes these molecules effective reagents as DNA vaccines, multimeric staining reagents to enumerate CD8 T cells, and probes of lymphocyte biology.

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