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. 2009 Nov:Chapter 17:17.5.1-17.5.17.
doi: 10.1002/0471142735.im1705s87.

Preparation of stable single-chain trimers engineered with peptide, beta2 microglobulin, and MHC heavy chain

Ted Hansen  1 , Y Y Lawrence Yu  1 , Daved H Fremont  1
Affiliations

Preparation of stable single-chain trimers engineered with peptide, beta2 microglobulin, and MHC heavy chain

Ted Hansen et al. Curr Protoc Immunol. 2009 Nov.

Abstract

This unit describes a method for constructing a class I MHC molecule with a bound peptide as a single polypeptide chain, termed SCT, for single chain trimer. The component organization of the SCT appears to be widely applicable to different mouse or human MHC class I isotypes bound by different antigenic peptides. The enhanced peptide occupancy afforded by the SCT format makes these molecules effective reagents as DNA vaccines, multimeric staining reagents to enumerate CD8 T cells, and probes of lymphocyte biology.

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References

Literature Cited

    1. Bloch, K.D. 1995. Mapping by multiple endonuclease digestions. Curr. Protoc. Mol. Biol. 13:3.2.1-3.2.5.
    1. Choudhuri, K., Wiseman, D., Brown, M.H., Gould, K., and van der Merwe, P.A. 2005. T-cell receptor triggering is critically dependent on the dimensions of its peptide-MHC ligand. Nature 436:578-582.
    1. Crew, M.D., Cannon, M.J., Phanavanh, B., and Garcia-Borges, C.N. 2005. An HLA-E single chain trimer inhibits human NK cell reactivity towards porcine cells. Mol. Immunol. 42:1205-1214.
    1. Fremont, D.H., Hendrickson, W.A., Marrack, P., and Kappler, J. 1996. Structures of an MHC class II molecule with covalently bound single peptides. Science 272:1001-1004.
    1. Fremont, D.H., Stura, E.A., Matsumura, M., Peterson, P.A., and Wilson, I.A. 1995. Crystal structure of an H-2Kb-ovalbumin peptide complex reveals the interplay of primary and secondary anchor positions in the major histocompatibility complex binding groove. Proc. Natl. Acad. Sci. U.S.A. 92:2479-2483.

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