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Review
. 2009 Sep 27;88(6):753-6.
doi: 10.1097/TP.0b013e3181b443ae.

Successful clinical islet isolation using a GMP-manufactured collagenase and neutral protease

Gregory L Szot  1 , Michael R Lee, Mohammad M Tavakol, Jiena Lang, Florinna Dekovic, Robert K Kerlan, Peter G Stock, Andrew M Posselt
Affiliations
Review

Successful clinical islet isolation using a GMP-manufactured collagenase and neutral protease

Gregory L Szot et al. Transplantation. .

Abstract

In 2007, the islet community was notified that the collagenase product most commonly used for human islet isolations contained bovine neural tissue contaminants. To minimize this potential hazard, we adapted our human islet processing procedure to use a GMP-manufactured, bovine neural tissue-free collagenase blend. Here, we describe the factors that we consider most important for achieving reproducible and clinically useable islet isolations using this product.

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References

    1. Fiorina P, Shapiro AMJ, Ricordi C, Secchi A. The clinical impact of islet transplantation. Am J Transplant. 2008;8:1990. - PubMed
    1. Fetterhoff TJ, Cavanagh TJ, Wile KJ, et al. Human pancreatic dissociation using a purified enzyme blend. Transplant Proc. 1995;27:3282. - PubMed
    1. Wile KJ, Fetterhoff TJ, Cavanagh TJ, Wright MJ. Image analysis of human pancreatic islets: a time-course approach to the evaluation of pancreatic dissociation. Transplant Proc. 1995;27:3246. - PubMed
    1. Shapiro AM, Lakey JR, Ryan EA, Korbutt GS, Toth E, Warnock GL, Kneteman NM, Rajotte RV. Islet transplantation in seven patients with type 1 diabetes mellitus using a glucocorticoid-free immunosuppressive regimen. N Engl J Med. 2000 Jul 27;343(4):230. - PubMed
    1. Tosoa C, Oberholzer J, Ris F, Triponez F, Bucher P, Demirag A, Andereggen E, Buehler L, Cretina N, Fourniera B, Majnoa P, Hong Y, Lou J, Morel P. Factors affecting human islet of Langerhans isolation yields. Transplantation Proceedings. 2002;34:3. - PubMed

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