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. 2010 Jun;136(6):855-62.
doi: 10.1007/s00432-009-0726-x. Epub 2009 Nov 17.

Differential expression of miRNA patterns in renal cell carcinoma and nontumorous tissues

Zhengjun Yi  1 , Yurong Fu, Shushu Zhao, Xuguang Zhang, Chuanxiang Ma
Affiliations

Differential expression of miRNA patterns in renal cell carcinoma and nontumorous tissues

Zhengjun Yi et al. J Cancer Res Clin Oncol. 2010 Jun.

Abstract

Introduction: MiRNAs are short single stranded RNAs that are associated with gene regulation at the transcriptional and translational level. Changes in their expression were found in a variety of human cancers. In the present study, we aimed to investigate expression profiles of miRNA (miRNA) in the clear cell subtype of kidney cancer and to develop further understanding of the molecular mechanisms involved in the pathogenesis of renal cell carcinoma.

Materials and methods: We analyzed the miRNA expression profiles in 30 pairs of renal cell carcinoma and adjacent nontumorous tissue (NT), using a mammalian miRNA microarray containing whole human mature and precursor miRNA sequences. Real-time quantitative PCR was performed to confirm the array results.

Results: The miRNA microarray chip analysis identified 86 miRNAs differentially expressed in renal cell carcinoma tissues and a total of 38 miRNAs exhibited higher expression in the renal cell carcinoma samples than that in the NT samples, while 48 miRNA demonstrated lower expression in the renal cell carcinoma samples than that in the NT samples.

Conclusion: Quantitative real-time PCR analysis confirmed microarray data. The report supports that many miRNA expressions were altered in renal carcinoma, whose expression profiling may provide a useful clue for the pathophysiology research. However, further longer-term researches are required to investigate the relationship between miRNA and renal carcinoma as well as their role in carcinogenesis.

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Figures

Fig. 1
Fig. 1
Histopathologic image of cell carcinoma of the nephrectomy specimen. Tissues were collected at the indicated time, fixed in l0% neutral buffered formalin, embedded in paraffin and then stained with hematoxylin and eosin (H&E) for histology examination. Data shown were a representative experiment (magnification ×200)
Fig. 2
Fig. 2
Hierarchical clustering of miRNA in kidney tissue samples. Kidney tissue samples were clustered according to the expression profile of 86 differentially expressed miRNAs between tumor tissue and normal tissue. Data from each miRNA were median centered. Samples are in columns and miRNAs in rows. N normal, T tumor. The P values for these miRNAs were less than 0.05 in tumor tissue compared with normal tissue
Fig. 3
Fig. 3
Confirmation miRNA expression by quantitative real-time PCR. Quantitative real-time PCR analysis confirmed microarray data: miR-16 and miR-451 were up-regulated; miR-141 and miR-200c were down-regulated in kidney cancer tissue. The experiment was conducted in triplicate. # P < 0.05 versus normal
See this image and copyright information in PMC

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