Transcriptome profiling of human pre-implantation development
- PMID: 19924284
- PMCID: PMC2773928
- DOI: 10.1371/journal.pone.0007844
Transcriptome profiling of human pre-implantation development
Abstract
Background: Preimplantation development is a crucial step in early human development. However, the molecular basis of human preimplantation development is not well known.
Methodology: By applying microarray on 397 human oocytes and embryos at six developmental stages, we studied the transcription dynamics during human preimplantation development.
Principal findings: We found that the preimplantation development consisted of two main transitions: from metaphase-II oocyte to 4-cell embryo where mainly the maternal genes were expressed, and from 8-cell embryo to blastocyst with down-regulation of the maternal genes and up-regulation of embryonic genes. Human preimplantation development proved relatively autonomous. Genes predominantly expressed in oocytes and embryos are well conserved during evolution.
Significance: Our database and findings provide fundamental resources for understanding
Conflict of interest statement
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References
-
- Wang QT, Piotrowska K, Ciemerych MA, Milenkovic L, Scott MP, et al. A genome-wide study of gene activity reveals developmental signaling pathways in the preimplantation mouse embryo. Dev Cell. 2004;6:133–144. - PubMed
-
- Hamatani T, Carter MG, Sharov AA, Ko MS. Dynamics of global gene expression changes during mouse preimplantation development. Dev Cell. 2004;6:117–131. - PubMed
-
- Mamo S, Sargent CA, Affara NA, Tesfaye D, El-Halawany N, et al. Transcript profiles of some developmentally important genes detected in bovine oocytes and in vitro-produced blastocysts using RNA amplification and cDNA microarrays. Reprod Domest Anim. 2006;41:527–534. - PubMed
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