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. 1991 Feb 1;88(3):1004-8.
doi: 10.1073/pnas.88.3.1004.

Specific initiation by RNA polymerase I in a whole-cell extract from yeast

M C Schultz  1 , S Y Choe, R H Reeder
Affiliations

Specific initiation by RNA polymerase I in a whole-cell extract from yeast

M C Schultz et al. Proc Natl Acad Sci U S A. .

Abstract

A protocol is described for making a soluble whole-cell extract from yeast (Saccharomyces cerevisiae) that supports active and specific transcription initiation by RNA polymerases I, II, and III. Specific initiation by polymerase I decreases in high-density cultures, paralleling the decrease in abundance of the endogenous 35S rRNA precursor. This extract should be useful for studying the molecular mechanisms that regulate rRNA transcription in yeast.

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