Genome-wide association studies: quality control and population-based measures

Abstract

Genome-wide association studies, using hundreds of thousands of single-nucleotide polymorphism (SNP) markers, have become a standard approach for identifying disease susceptibility genes. The change in the technology poses substantial computational and statistical challenges that have been addressed in the quality control, imputation, and population-based measure groups of the Genetic Analysis Workshop 16. The computational challenges pertain to efficient memory management and computational speed of the statistical procedures, and we discuss an approach for efficient SNP storage. Accuracy and computational speed is relevant for genotype calling, and the results from a comparison of three calling algorithms are discussed. The first statistical challenge is related to statistical quality control, and we discuss two novel quality control procedures. These low-level analyses have an effect on subsequent preparatory steps for high-level analyses, e.g., the quality of genotype imputation approaches. After the conduct of a genome-wide association study with successful replication and/or validation, measures of diagnostic accuracy, including the area under the curve, are investigated. The area under the curve can be constructed from summary data in some situations. Finally, we discuss how the population-attributable risk of a genetic variant that is only measured in a reference data set can be determined.

Figure 1

Succession of design, experimental and data analysis steps in a genome-wide association and subsequent studies. Adapted from Ziegler et al. [2008].