[Expression of genes connected with nuclear factor kappa B (NFkappaB) estimated by oligonucleotide microarray analysis HG-U133A in patients with acute myocardial infarction]

Abstract

Atherosclerosis is a permanently progressive chronic inflammatory disorder which nuclear factor kappaKB (NFkappaB) is involved. Therefore NFkappaB has become integral aspect of atherogenesis and its complications.

The aim of the study: Estimation of genes expression involved in NFkappaB signaling pathway and separation genes differentiate patients with acute myocardial infarction from healthy subjects.

Material and methods: The examination was assess using the Affymetrix HG-U133A oligonucleotide microarray. Differentiating genes were determined using Bland-Altman graph analysis. Patients wasn't treated due to cardiac diseases before. All patients were subjected to 12-lead ECG, 2-D echocardiography, coronarography and laboratory studies including cardiac troponin, CK and CK-MB. The healthy individuals were subjected to coronarography and computed tomography (calcium score)--coronary artery disease was out of the question.

Results: Hierarchical clusterization has demonstrated that the genes expression of patients with acute myocardial infarction was different from healthy individuals. It also demonstrated that the individual groups are homogeneous, especially the group of patients with acute myocardial infarction, regardless of diagnosis, number of risk factors and progression of coronary artery disease. Further Bland-Altman graph analysis showed three important differentiating genes: TLR2, TNFRSF1A i IKBKAP.

Conclusions: Our results confirmed the share of genes involved in NFkappaB signaling pathway in acute complications of atherosclerosis. Noticed differences in genes expression of patients with acute myocardial infarction and healthy subjects can show important role isolated differentiating genes in destabilization of atherogenic plaque and acute myocardial infarction occurrence.