Chemosystematic value of the essential oil composition of Thuja species cultivated in Poland-antimicrobial activity

Abstract

In the framework of the correlation between chemotaxonomy and chemical analysis studies, the chemical composition of the essential oils of four varieties of Thuja species cultivated in Poland - T. occidentalis 'globosa', T. occidentalis 'aurea', T. plicata and T. plicata 'gracialis' - were investigated by GC and GC-MS. Thirty-one compounds were identified from T. occidentalis 'globosa', representing 96.92% of the total oil; twenty-seven from T. occidentalis 'aurea' (94.34%); thirty-one from T. plicata (94.75%); and thirty compounds from T. plicata 'gracialis' (96.36%). The main constituents in all samples were the monoterpene ketones alpha- and beta-thujone, fenchone and sabinene, as well as the diterpenes beyerene and rimuene. The chemosystematic value of the total ketone content of all samples (which varied from 54.30-69.18%) has been discussed and investigated. The constituents, beyerene and the mixture of alpha- and beta-thujone, were isolated from the oils and tested against six Gram-positive and -negative bacteria and three pathogenic fungi. The oils of the two T. plicata species exhibited significant antimicrobial activity, while the mixture of alpha- and beta-thujone showed very strong activity as well.