A distinct vesicle population targets membranes and pore complexes to the nuclear envelope in Xenopus eggs
- PMID: 1993730
- PMCID: PMC2288859
- DOI: 10.1083/jcb.112.4.545
A distinct vesicle population targets membranes and pore complexes to the nuclear envelope in Xenopus eggs
Abstract
Extracts from Xenopus eggs capable of nuclear envelope assembly in vitro were fractionated by differential and density gradient centrifugation. Nuclear envelope assembly was found to require soluble components in the cytosol and two distinct particulate fractions, which we have called nuclear envelope precursor fractions A and B (NEP-A and NEP-B). Both NEP-A and NEP-B are sensitive to treatments with trypsin, sodium carbonate, and detergents, but can be distinguished from each other by their sensitivities to high salt and N-ethylmaleimide and by their levels of alpha-glucosidase activity. Vesicles in NEP-B bind to chromatin, whereas those in NEP-A do not. NEP-B may therefore be involved in the targeting of membranes to the surface of the chromatin, whereas NEP-A may provide a pool of vesicles that contributes many of the nuclear envelope membranes. NEP-B may also play a role in the assembly of nuclear pore complexes because the density of nuclear pores in the resulting envelope is dependent on the ratio of NEP-B to NEP-A in the reconstituted extract.
Similar articles
-
Regulation of nuclear envelope precursor functions during cell division.J Cell Sci. 1992 Jun;102 ( Pt 2):273-84. doi: 10.1242/jcs.102.2.273. J Cell Sci. 1992. PMID: 1400633
-
Characterization of the membrane binding and fusion events during nuclear envelope assembly using purified components.J Cell Biol. 1992 Jan;116(2):295-306. doi: 10.1083/jcb.116.2.295. J Cell Biol. 1992. PMID: 1730757 Free PMC article.
-
NEP-A and NEP-B both contribute to nuclear pore formation in Xenopus eggs and oocytes.J Cell Sci. 2008 Mar 1;121(Pt 5):706-16. doi: 10.1242/jcs.019968. Epub 2008 Feb 12. J Cell Sci. 2008. PMID: 18270266
-
Analysis of nuclear envelope assembly using extracts of Xenopus eggs.Methods Cell Biol. 1998;53:367-95. doi: 10.1016/s0091-679x(08)60887-0. Methods Cell Biol. 1998. PMID: 9348517 Review. No abstract available.
-
Spatial and temporal control of nuclear envelope assembly by Ran GTPase.Symp Soc Exp Biol. 2004;(56):193-204. Symp Soc Exp Biol. 2004. PMID: 15565882 Review.
Cited by
-
Reconstitution of ionic channels from inner and outer membranes of mammalian cardiac nuclei.Biophys J. 1996 Feb;70(2):703-14. doi: 10.1016/S0006-3495(96)79610-8. Biophys J. 1996. PMID: 8789087 Free PMC article.
-
Integral membrane proteins of the nuclear envelope are dispersed throughout the endoplasmic reticulum during mitosis.J Cell Biol. 1997 Jun 16;137(6):1199-210. doi: 10.1083/jcb.137.6.1199. J Cell Biol. 1997. PMID: 9182656 Free PMC article.
-
Direct membrane protein-DNA interactions required early in nuclear envelope assembly.J Cell Biol. 2006 May 22;173(4):469-76. doi: 10.1083/jcb.200512078. J Cell Biol. 2006. PMID: 16717124 Free PMC article.
-
A role for nuclear lamins in nuclear envelope assembly.J Cell Biol. 2001 Jul 9;154(1):61-70. doi: 10.1083/jcb.200101025. J Cell Biol. 2001. PMID: 11448990 Free PMC article.
-
Emerin Is Required for Proper Nucleus Reassembly after Mitosis: Implications for New Pathogenetic Mechanisms for Laminopathies Detected in EDMD1 Patients.Cells. 2019 Mar 13;8(3):240. doi: 10.3390/cells8030240. Cells. 2019. PMID: 30871242 Free PMC article.