Comparative Study
doi: 10.1128/JCM.01790-09.
Epub 2009 Nov 25.
Enhanced detection of enteroviruses in clinical samples by reverse transcription-PCR using complementary locked primer technology
Affiliations
- PMID: 19940056
- PMCID: PMC2815621
- DOI: 10.1128/JCM.01790-09
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Comparative Study
Enhanced detection of enteroviruses in clinical samples by reverse transcription-PCR using complementary locked primer technology
J Clin Microbiol.
2010 Feb.
Abstract
To increase detection sensitivity, we modified primers using complementary locked primer (CLP) technology. The sensitivity of the reverse transcription-PCR (RT-PCR) with CLP-modified primers was 10- to 100-fold higher than that of RT-PCR without these primers. CLP-modified primers can increase sensitivity, providing a widely accessible method for molecular diagnosis.
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