doi: 10.1111/j.1432-1033.1977.tb11780.x.
DNA unwinding enzyme II of Escherichia coli. 1. Purification and characterization of the ATPase activity
- PMID: 199440
- DOI: 10.1111/j.1432-1033.1977.tb11780.x
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DNA unwinding enzyme II of Escherichia coli. 1. Purification and characterization of the ATPase activity
Eur J Biochem.
.
Free article
Abstract
A DNA-stimulated ATP-gamma-phosphohydrolase of molecular weight 75000 was purified from Escherichia coli cells. The ATPase, a globular molecule (identical probably with an ATPase described previously by Richet and Kohiyama in 1976) shows specificity for adenine nucleotides, it prefers single-stranded DNA as the cofactor, it exhibits a complicated mode of response to variations of the cofacter concentration and it is devoid of nuclease activity. Preparations derived from rep3 mutant cells yield widely varying amounts of an apparently normal ATPase.
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