Review
doi: 10.1007/s00253-009-2349-1.
Epub 2009 Nov 28.
Methodologies to increase the transformation efficiencies and the range of bacteria that can be transformed
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- PMID: 19946685
- DOI: 10.1007/s00253-009-2349-1
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Review
Methodologies to increase the transformation efficiencies and the range of bacteria that can be transformed
Appl Microbiol Biotechnol.
2010 Feb.
Abstract
Methods for transfer of exogenous DNA into cells are essential for genetics and molecular biology, and the lack of effective methods hampers research on many different species of bacteria which have shown to be particularly recalcitrant to transformation. This review presents the progress on the development of methods for artificial transformation of bacteria with emphasis on different methodologies and the range of bacteria that can be transformed. The methods' strengths and weaknesses are described.
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