Arabidopsis N-MYC DOWNREGULATED-LIKE1, a positive regulator of auxin transport in a G protein-mediated pathway
- PMID: 19948787
- PMCID: PMC2798320
- DOI: 10.1105/tpc.109.065557
Arabidopsis N-MYC DOWNREGULATED-LIKE1, a positive regulator of auxin transport in a G protein-mediated pathway
Abstract
Root architecture results from coordinated cell division and expansion in spatially distinct cells of the root and is established and maintained by gradients of auxin and nutrients such as sugars. Auxin is transported acropetally through the root within the central stele and then, upon reaching the root apex, auxin is transported basipetally through the outer cortical and epidermal cells. The two Gbetagamma dimers of the Arabidopsis thaliana heterotrimeric G protein complex are differentially localized to the central and cortical tissues of the Arabidopsis roots. A null mutation in either the single beta (AGB1) or the two gamma (AGG1 and AGG2) subunits confers phenotypes that disrupt the proper architecture of Arabidopsis roots and are consistent with altered auxin transport. Here, we describe an evolutionarily conserved interaction between AGB1/AGG dimers and a protein designated N-MYC DOWNREGULATED-LIKE1 (NDL1). The Arabidopsis genome encodes two homologs of NDL1 (NDL2 and NDL3), which also interact with AGB1/AGG1 and AGB1/AGG2 dimers. We show that NDL proteins act in a signaling pathway that modulates root auxin transport and auxin gradients in part by affecting the levels of at least two auxin transport facilitators. Reduction of NDL family gene expression and overexpression of NDL1 alter root architecture, auxin transport, and auxin maxima. AGB1, auxin, and sugars are required for NDL1 protein stability in regions of the root where auxin gradients are established; thus, the signaling mechanism contains feedback loops.
Figures









Similar articles
-
N-MYC down-regulated-like proteins regulate meristem initiation by modulating auxin transport and MAX2 expression.PLoS One. 2013 Nov 4;8(11):e77863. doi: 10.1371/journal.pone.0077863. eCollection 2013. PLoS One. 2013. PMID: 24223735 Free PMC article.
-
Glucose attenuation of auxin-mediated bimodality in lateral root formation is partly coupled by the heterotrimeric G protein complex.PLoS One. 2010 Sep 17;5(9):e12833. doi: 10.1371/journal.pone.0012833. PLoS One. 2010. PMID: 20862254 Free PMC article.
-
Gβγ dimers mediate low K+ stress-inhibited root growth via modulating auxin redistribution in Arabidopsis.Plant Cell Environ. 2024 Dec;47(12):5039-5052. doi: 10.1111/pce.15075. Epub 2024 Aug 13. Plant Cell Environ. 2024. PMID: 39136400
-
Spatiotemporal asymmetric auxin distribution: a means to coordinate plant development.Cell Mol Life Sci. 2006 Dec;63(23):2738-54. doi: 10.1007/s00018-006-6116-5. Cell Mol Life Sci. 2006. PMID: 17013565 Free PMC article. Review.
-
Signalling Overlaps between Nitrate and Auxin in Regulation of The Root System Architecture: Insights from the Arabidopsis thaliana.Int J Mol Sci. 2020 Apr 20;21(8):2880. doi: 10.3390/ijms21082880. Int J Mol Sci. 2020. PMID: 32326090 Free PMC article. Review.
Cited by
-
Necrotroph attacks on plants: wanton destruction or covert extortion?Arabidopsis Book. 2010;8:e0136. doi: 10.1199/tab.0136. Epub 2010 Aug 10. Arabidopsis Book. 2010. PMID: 22303261 Free PMC article.
-
Interaction between glucose and brassinosteroid during the regulation of lateral root development in Arabidopsis.Plant Physiol. 2015 May;168(1):307-20. doi: 10.1104/pp.114.256313. Epub 2015 Mar 25. Plant Physiol. 2015. PMID: 25810094 Free PMC article.
-
N-MYC down-regulated-like proteins regulate meristem initiation by modulating auxin transport and MAX2 expression.PLoS One. 2013 Nov 4;8(11):e77863. doi: 10.1371/journal.pone.0077863. eCollection 2013. PLoS One. 2013. PMID: 24223735 Free PMC article.
-
The G Protein β-Subunit, AGB1, Interacts with FERONIA in RALF1-Regulated Stomatal Movement.Plant Physiol. 2018 Mar;176(3):2426-2440. doi: 10.1104/pp.17.01277. Epub 2018 Jan 4. Plant Physiol. 2018. PMID: 29301953 Free PMC article.
-
Arabidopsis heterotrimeric G protein β subunit, AGB1, regulates brassinosteroid signalling independently of BZR1.J Exp Bot. 2013 Aug;64(11):3213-23. doi: 10.1093/jxb/ert159. Epub 2013 Jun 27. J Exp Bot. 2013. PMID: 23814276 Free PMC article.
References
-
- Akgoz, M., Azpiazu, I., Kalyanaraman, V., and Gautam, N. (2002). Role of the G protein gamma subunit in beta gamma complex modulation of phospholipase Cβ function. J. Biol. Chem. 277 19573–19578. - PubMed
-
- Benjamins, R., Malenica, N., and Luschnig, C. (2005). Regulating the regulator: the control of auxin transport. Bioessays 27 1246–1255. - PubMed
-
- Benjamins, R., and Scheres, B. (2008). Auxin: The looping star in plant development. Annu. Rev. Plant Biol. 59 443–465. - PubMed
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Miscellaneous