A multiplex PCR assay for the direct identification of the capsular type (Ia to IX) of Streptococcus agalactiae
- PMID: 19958797
- DOI: 10.1016/j.mimet.2009.11.010
A multiplex PCR assay for the direct identification of the capsular type (Ia to IX) of Streptococcus agalactiae
Abstract
A multiplex PCR assay for the identification of serotypes Ia to IX of Streptococcus agalactiae was developed. By using a single PCR reaction containing a mix of 19 primers the assay identified each serotype by the analysis of the unique two or three bands pattern on agarose gel.
Copyright 2009 Elsevier B.V. All rights reserved.
Similar articles
-
Multiplex PCR assay for rapid and accurate capsular typing of group B streptococci.J Clin Microbiol. 2007 Jun;45(6):1985-8. doi: 10.1128/JCM.00159-07. Epub 2007 Mar 21. J Clin Microbiol. 2007. PMID: 17376884 Free PMC article.
-
Evaluation of a multiplex PCR-based reverse line blot-hybridization assay for identification of serotype and surface protein antigens of Streptococcus agalactiae.J Clin Microbiol. 2006 Oct;44(10):3822-5. doi: 10.1128/JCM.01232-06. J Clin Microbiol. 2006. PMID: 17021119 Free PMC article.
-
Molecular serotype identification of Streptococcus agalactiae of bovine origin by multiplex PCR-based reverse line blot (mPCR/RLB) hybridization assay.FEMS Microbiol Lett. 2006 Oct;263(2):236-9. doi: 10.1111/j.1574-6968.2006.00428.x. FEMS Microbiol Lett. 2006. PMID: 16978362
-
[Molecular genetic methods of identification of infectious bacterial agents].Voen Med Zh. 2014 Oct;335(10):50-4. Voen Med Zh. 2014. PMID: 25532312 Review. Russian.
-
Principles and applications of methods for DNA-based typing of microbial organisms.J Clin Microbiol. 1999 Jun;37(6):1661-9. doi: 10.1128/JCM.37.6.1661-1669.1999. J Clin Microbiol. 1999. PMID: 10325304 Free PMC article. Review. No abstract available.
Cited by
-
Analysis of the type II-A CRISPR-Cas system of Streptococcus agalactiae reveals distinctive features according to genetic lineages.Front Genet. 2015 Jun 15;6:214. doi: 10.3389/fgene.2015.00214. eCollection 2015. Front Genet. 2015. PMID: 26124774 Free PMC article.
-
Serotype and surface protein gene distribution of colonizing group B streptococcus in women in Egypt.Epidemiol Infect. 2014 Jan;142(1):208-10. doi: 10.1017/S0950268813000848. Epub 2013 Apr 8. Epidemiol Infect. 2014. PMID: 23561305 Free PMC article.
-
Is Penicillin Plus Gentamicin Synergistic against Clinical Group B Streptococcus isolates?: An In vitro Study.Front Microbiol. 2016 Oct 21;7:1680. doi: 10.3389/fmicb.2016.01680. eCollection 2016. Front Microbiol. 2016. PMID: 27818657 Free PMC article.
-
Group B streptococcus serotype prevalence in reproductive-age women at a tertiary care military medical center relative to global serotype distribution.BMC Infect Dis. 2010 Nov 24;10:336. doi: 10.1186/1471-2334-10-336. BMC Infect Dis. 2010. PMID: 21106080 Free PMC article.
-
Association between functional antibody against Group B Streptococcus and maternal and infant colonization in a Gambian cohort.Vaccine. 2017 May 19;35(22):2970-2978. doi: 10.1016/j.vaccine.2017.04.013. Epub 2017 Apr 24. Vaccine. 2017. PMID: 28449969 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources