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Comparative Study
. 2010 Dec;34(8):1327-32.
doi: 10.1007/s00264-009-0917-8. Epub 2009 Dec 5.

Potency of double-layered poly L-lactic acid scaffold in tissue engineering of tendon tissue

Atsuyuki Inui  1 , Takeshi Kokubu, Takeshi Makino, Issei Nagura, Narikazu Toyokawa, Ryosuke Sakata, Masaru Kotera, Takashi Nishino, Hiroyuki Fujioka, Masahiro Kurosaka
Affiliations
Comparative Study

Potency of double-layered poly L-lactic acid scaffold in tissue engineering of tendon tissue

Atsuyuki Inui et al. Int Orthop. 2010 Dec.

Abstract

A successful scaffold for use in tendon tissue engineering requires a high affinity for living organisms and the ability to maintain its mechanical strength until maturation of the regenerated tissue. We compared two types of poly(L-lactic acid) (PLLA) scaffolds for use in tendon regeneration, a plain-woven PLLA fabric (fabric P) with a smooth surface only and a double layered PLLA fabric (fabric D) with a smooth surface on one side and a rough (pile-finished) surface on the other side. These two types of fabric were implanted into the back muscles of rabbits and evaluated at three and six weeks after implantation. Histological examination showed collagen tissues were highly regenerated on the rough surface of fabric D. On the other hand, liner cell attachment was seen in the smooth surface of fabric P and fabric D. The total DNA amount was significantly higher in fabric D. Additionally, mechanical examination showed fabric P had lost its mechanical strength by six weeks after implantation, while the strength of fabric D was maintained. Fabric D had more cell migration on one side and less cell adhesion on the other side and maintained its initial strength. Thus, a novel form of double-layered PLLA fabric has the potential to be used as a scaffold in tendon regeneration.

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Figures

Fig. 1
Fig. 1
Microscopic appearance of PLLA fabric (scale bar represents 1 mm). a Fabric P. b Smooth side of fabric D. c Pile-finished side of fabric D
Fig. 2
Fig. 2
Microscopic appearance of two types of PLLA fabric in the back muscle of the rabbits (H-E staining). Linear cell attachment was seen in both fabrics. Cell migration was not seen interstices of the fibres in fabric P. On the other hand, a large amount of cell migration was seen internally on the pile-finished side of fabric D (scale bar represent 100 μm). a Fabric P after three weeks implantation. b Fabric D after three weeks implantation. c Fabric P after six weeks implantation. d Fabric D after six weeks implantation
Fig. 3
Fig. 3
Azan staining of fabrics at six weeks implantation. Blue stained area shows collagenous tissue. Both fabrics were covered with collagenous tissue. More collagenous tissue was seen on the pile-finished side of fabric D (scale bar represent 100 μm). a Fabric P. b Fabric D (the left side of the figure is the smooth side of the fabric and the right side is the pile-finished side)
Fig. 4
Fig. 4
Total DNA amount. Fabric D showed a significantly higher amount of total DNA. a Fabric D. b Fabric P
Fig. 5
Fig. 5
Mechanical analysis. The change ratio of ultimate failure load or energy was calculated by dividing the measured value of each time point by the value of preoperative data. Both fabrics lost their mechanical strength by six weeks after implantation; however, no significant loss was seen in fabric D. a Ultimate failure load of fabric. b Energy of fabric
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