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. 2008 Dec;12(6):343-7.
doi: 10.4196/kjpp.2008.12.6.343. Epub 2008 Dec 31.

The Effect of Caffeic Acid on Wound Healing in Skin-incised Mice

Ho Sun Song  1 , Tae Wook Park, Uy Dong Sohn, Yong Kyoo Shin, Byung Chul Choi, Chang Jong Kim, Sang Soo Sim
Affiliations

The Effect of Caffeic Acid on Wound Healing in Skin-incised Mice

Ho Sun Song et al. Korean J Physiol Pharmacol. 2008 Dec.

Abstract

This study was carried out to investigate the wound healing effect of caffeic acid in skin-incised mice. Caffeic acid showed significant effects on anti-inflammatory activity and wound healing, such as myeloperoxidase activity, lipid peroxidation, phospholipase A(2) activity and collagen-like polymer synthesis, in incised-wound tissue. On the other hand, it significantly stimulated collagen-like polymer synthesis in NIH 3T3 fibroblast cells, while inhibited both silica-induced reactive oxygen species generation and melittin-induced arachidonic acid release and PGE(2) production in Raw 264.7 cells, and histamine release in RBL 2H3 cells stimulated by melittin or arachidonic acid. Therefore, caffeic acid appears to have a potent antioxidant and anti-inflammatory effect in cell culture system, which may be related to wound healing in skin-incised mice.

Keywords: Arachidonic acid; Caffeic acid; Collagen; Histamine; Wound healing.

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Figures

Fig. 1
Fig. 1
Effect of caffeic acid on wound healing in skin-incised mice. Two cm long incision wound perforating the skin was made on the dorsal skin of mice. Mice were orally treated daily with 1% carboxymethyl cellulose solution (CMC; Control) and 10 mg/kg caffeic acid in 1% CMC solution during the study period. On days 5, 10 and 15, zones of about 1mm in thickness were taken from the edges of the wounds for biochemical analysis, such as lipid peroxidation (A), phospholipase A2 (B), myeloperoxidase (C) and collagen-like polymer (D). Results are means±SD from 5 mice. *Significantly different from control (p<0.05).
Fig. 2
Fig. 2
Effect of caffeic acid on collagen-like polymer production in NIH 3T3 cells. NIH 3T3 cells were incubated with caffeic acid at 37℃ for 48 h. Control was the cells treated with 1% DMSO. Results are means±SD from 5 separate experiments. *Significantly different from control (p<0.05).
Fig. 3
Fig. 3
Effect of caffeic acid on reactive oxygen species (ROS) generation in Raw 264.7 cells. DCF-loaded Raw 264.7 cells were preincubated with caffeic acid and stimulated with 1 mg/ml silica at 37℃ for 30 min. Results are means±SD from 5 separate experiments. *Significantly different from control (p<0.05).
Fig. 4
Fig. 4
Effect of caffeic acid on melittin-induced arachidonic acid release and PGE2 production in Raw 264.7 cells. The cells were stimulated with 0.5 µM melittin in the presence or absence of caffeic acid at 37℃ for 30 min. Control was treated with 1% DMSO in the absence of melittin. Intracellular amount of PGE2 was measured using PGE2 assay kit. Results are means±SD from 4 separate experiments. *Significantly different from control (p<0.05).
Fig. 5
Fig. 5
Effect of caffeic acid on melittin or arachidonic acid-induced histamine release in RBL 2H3 cells. The cells were stimulated with 0.5 µM melittin (A) or 100 µM arachidonic acid (AA; B) in the presence or absence of caffeic acid at 37℃ for 30 min. Control was treated with 1% DMSO in the absence of melittin or arachidonic acid. Results are means±SD from 5 separate experiments. *Significantly different from control (p<0.05).
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