Steady-state staphylococcal enterotoxin type C mRNA is affected by a product of the accessory gene regulator (agr) and by glucose
- PMID: 1997441
- PMCID: PMC258352
- DOI: 10.1128/iai.59.3.955-962.1991
Steady-state staphylococcal enterotoxin type C mRNA is affected by a product of the accessory gene regulator (agr) and by glucose
Abstract
The effects of the accessory gene regulator (agr) and glucose on staphylococcal enterotoxin type C (SEC) gene (sec+) expression were examined. For the agr studies, a Tn551 insertionally inactivated agr was transferred into two different sec+ Staphylococcus aureus strains. Western blot (immunoblot) analysis showed that each of the sec+ Agr- derivatives produced less extracellular SEC than their Agr+ parent strains. Analysis of Northern (RNA) blots was consistent with at least part of the agr effect being at the level of steady-state sec+ mRNA. We examined the glucose effect on sec+ expression by utilizing both a fermentor system with a completely defined amino acid-containing medium in which the pH of the medium was maintained at 6.5 and a shake flask system with a complex medium in which the pH was allowed to fluctuate during bacterial growth. In both systems, samples from the cultures containing glucose had less extracellular SEC and less steady-state sec+ mRNA compared with the control cultures which lacked glucose. An intact agr was not required for the glucose effect on sec+ expression; MJB407, an Agr- sec+ strain, produced more SEC and had more steady-state sec+ mRNA when grown in medium that lacked glucose compared with medium that contained glucose.
Similar articles
-
Alkaline pH decreases expression of the accessory gene regulator (agr) in Staphylococcus aureus.J Bacteriol. 1992 Aug;174(15):5095-100. doi: 10.1128/jb.174.15.5095-5100.1992. J Bacteriol. 1992. PMID: 1629166 Free PMC article.
-
Staphylococcal enterotoxin A gene (sea) expression is not affected by the accessory gene regulator (agr).Infect Immun. 1993 Jan;61(1):356-9. doi: 10.1128/iai.61.1.356-359.1993. Infect Immun. 1993. PMID: 7678101 Free PMC article.
-
High sodium chloride concentrations inhibit staphylococcal enterotoxin C gene (sec) expression at the level of sec mRNA.Infect Immun. 1993 Apr;61(4):1581-5. doi: 10.1128/iai.61.4.1581-1585.1993. Infect Immun. 1993. PMID: 8454367 Free PMC article.
-
Glucose and nonmaintained pH decrease expression of the accessory gene regulator (agr) in Staphylococcus aureus.Infect Immun. 1992 Aug;60(8):3381-8. doi: 10.1128/iai.60.8.3381-3388.1992. Infect Immun. 1992. PMID: 1639506 Free PMC article.
-
Staphylococcus aureus and Staphylococcus epidermidis peptide pheromones produced by the accessory gene regulator agr system.Peptides. 2001 Oct;22(10):1603-8. doi: 10.1016/s0196-9781(01)00495-8. Peptides. 2001. PMID: 11587788 Review.
Cited by
-
Characterization of the promoter elements for the staphylococcal enterotoxin D gene.J Bacteriol. 2000 Apr;182(8):2321-5. doi: 10.1128/JB.182.8.2321-2325.2000. J Bacteriol. 2000. PMID: 10735879 Free PMC article.
-
Glucose-related dissociation between icaADBC transcription and biofilm expression by Staphylococcus epidermidis: evidence for an additional factor required for polysaccharide intercellular adhesin synthesis.J Bacteriol. 2003 May;185(9):2879-86. doi: 10.1128/JB.185.9.2879-2886.2003. J Bacteriol. 2003. PMID: 12700267 Free PMC article.
-
Alkaline pH decreases expression of the accessory gene regulator (agr) in Staphylococcus aureus.J Bacteriol. 1992 Aug;174(15):5095-100. doi: 10.1128/jb.174.15.5095-5100.1992. J Bacteriol. 1992. PMID: 1629166 Free PMC article.
-
Accessory gene regulator control of staphyloccoccal enterotoxin d gene expression.J Bacteriol. 2004 Mar;186(6):1793-801. doi: 10.1128/JB.186.6.1793-1801.2004. J Bacteriol. 2004. PMID: 14996810 Free PMC article.
-
Role of sodium salicylate in Staphylococcus aureus quorum sensing, virulence, biofilm formation and antimicrobial susceptibility.Front Microbiol. 2022 Aug 1;13:931839. doi: 10.3389/fmicb.2022.931839. eCollection 2022. Front Microbiol. 2022. PMID: 35992652 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources