[Structure and function of virus-induced antigens in cultured cells infected with Merek's disease and turkey herpes viruses. II. Isolation of intracellular antigens from infected cells (author's transl)]

Abstract

Virus-induced antigens were isolated from MDV and HVT-infected cells by salt extraction with 3M KCl and purified by Con.A chromatography and isoelectric focusing. Electrophoretic analysis of 35S-methionine, 3H-fucose and 32P-orthophosphate labeled antigens revealed 7 different polypeptides, two of them containing labeled carbohydrates, and one phospholipid component. The isolated virus-induced antigens from infected cells were identified as serologically active membrane complexes carrying common antigenic determinants of MDV and HVT. They were able to bind to virus-neutralizing immunglobulins as shown by antibody binding tests. Summarizing the presented analytic and serologic findings it was proposed to classify MDV and HVT as different serotypes of a common Marek's disease virus group.