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. 1991 Jan 15;97(2):267-72.
doi: 10.1016/0378-1119(91)90061-f.

Cloning of trichosanthin cDNA and its expression in Escherichia coli

P C Shaw  1 , M H Yung, R H Zhu, W K Ho, T B Ng, H W Yeung
Affiliations

Cloning of trichosanthin cDNA and its expression in Escherichia coli

P C Shaw et al. Gene. .

Abstract

Several cDNA clones coding for trichosanthin (TCS) have been isolated from a cDNA library prepared from the poly(A)+RNA of the root tuber of Trichosanthes kirilowii Maximowicz. The nucleotide sequence codes for a protein of 289 amino acids (aa) including a putative signal peptide of 23 aa and an extra 19 aa at the C terminus; the latter two have not been found in TCS obtained from the natural product [Collins et al., J. Biol. Chem. 265 (1990) 8665-8669]. Recombinant TCS (reTCS) was synthesized in Escherichia coli, in which the cDNA without the signal sequence was expressed under the control of the trc promoter; reTCS was detected by a rabbit anti-TCS antiserum.

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