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. 2010 Feb;80(2):209-11.
doi: 10.1016/j.mimet.2009.11.009. Epub 2009 Dec 6.

Improved detection of microbial DNA after bead-beating before DNA isolation

Richard de Boer  1 , Remco Peters, Sonja Gierveld, Tim Schuurman, Mirjam Kooistra-Smid, Paul Savelkoul
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Improved detection of microbial DNA after bead-beating before DNA isolation

Richard de Boer et al. J Microbiol Methods. 2010 Feb.

Abstract

Detection of microbial DNA in clinical samples by real-time PCR requires a universal extraction method with equally efficient lysis of cell walls of all possible microorganisms. We demonstrated that physical disruption by bead-beating with 0.1mm beads in combination with MagNA Pure DNA III extraction enhances microbial lysis of diverse Gram-positive microorganisms and may be used to optimize DNA extraction protocols in routine clinical diagnostics.

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