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. 2010 Feb;54(2):907-11.
doi: 10.1128/AAC.01080-09. Epub 2009 Dec 7.

Development of an allele-specific PCR for detection of the K65R resistance mutation in patients infected with subtype C human immunodeficiency virus type 1

Thomas A Toni  1 , Bluma G Brenner, Eugene L Asahchop, Michel Ntemgwa, Daniella Moisi, Mark A Wainberg
Affiliations

Development of an allele-specific PCR for detection of the K65R resistance mutation in patients infected with subtype C human immunodeficiency virus type 1

Thomas A Toni et al. Antimicrob Agents Chemother. 2010 Feb.

Abstract

The selection of drug-resistant variants of human immunodeficiency virus type 1 (HIV-1) is an impediment to the efficiency of antiretroviral (ARV) therapy. We have developed an allele-specific real-time PCR assay to explore the presence of K65R minority species among treated HIV-1 subtype B and C infections. Thirty HIV-1 subtype C- and 26 subtype B-infected patients lacking K65R as determined by conventional sequencing methods were studied, and viral minority species were found in four HIV-1 subtype C samples.

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Figures

FIG. 1.
FIG. 1.
Presence and absence of K65R as a minority species. We tested HIV-1 samples and standards for the presence of the K65R mutation as a minority species by using our novel AS-PCR assay as described in the text. (a) The amplification plots show that K65R was present in subtype C MJ4 K65R plasmid (107 copies/μl). The K65R curve crosses the threshold level earlier than that of the total virus population and of the wild type, indicating the presence of K65R only in the former. Positive results were obtained for each of the four patient samples tested under the same conditions, as shown in panels b, c, d, and e.
See this image and copyright information in PMC

References

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