[Attempts at specific immunoprophylaxis against herpes mammillitis]

Abstract

A live cell culture adsorbate vaccine was produced against herpes mammillitis in cattle. Comparative investigations were carried out on the immunogenic properties of four vaccines having different adjuvants--aluminum hydroxide, saponin, arlacell, and Freund's adjuvant. All tested vaccines proved harmless and immunogenic for sensitive cattle, the highest immunogenic effect being produced by the vaccine that contained aluminum hydroxide and Freund's adjuvant. The vaccinated animals reacted to immunization with the formation of specific antibodies that showed a highest titre between the 21st and 28th day following treatment. Immunity was checked through challenge on the 45th day, all immunized heifers being fully protected against Bovid herpesvirus infection 2 infection. When necessary the vaccine can be used successfully in a dose of 3-6 cm3, subcutaneously, in the region of the dewlap.