[Activity of enzymes of glycolysis and gluconeogenesis in primary hepatomas induced by diethylnitrosamine]

Abstract

Activities of the key enzymes of glycolysis and gluconeogenesis were studied in soluble fraction of primary hepatoma, induced by diethyl nitrosamine (DENA) in rats and mice of C3HA strain. Histological structure of the tumors studied showed that they belonged mainly to highly differentiated hepatocellular adenomas. Liver tissue of the animals which were not treated with DENA, was used as a control. The hexokinase activity was increased about 2-fold in the rat hepatomas and 3-fold--in the mice hepatomas. In primary hepatomas the glucokinase activity was unaltered or slightly decreased; the fructokinase activity was distinctly decreased (in several cases down to zero). The activity of key enzymes of gluconeogenesis--glucose-6-phosphatase and fructose-1,6-diphosphatase--was distinctly decreased, especially the activity of the former enzyme, which constituted in hepatomas about 1/6 of the activity found in liver tissue.