Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2009 Nov;9(4):335-41.
doi: 10.17305/bjbms.2009.2790.

The study of volume density of tracheal ganglions in vitro in new born babies with respiratory distress syndrome

Ragip Shabani  1 , Hilmi Islami, Sadi Bexheti, Fehmi Zeqiri, Ramadan Dacaj, Ilir Kurtishi, Naim Haliti, Ruke Beqiri, Labinot Shahini
Affiliations

The study of volume density of tracheal ganglions in vitro in new born babies with respiratory distress syndrome

Ragip Shabani et al. Bosn J Basic Med Sci. 2009 Nov.

Abstract

Volume density of respiratory organs was studied in vitro in newborn babies at different age of gestation (abort, immature, premature and mature) using stereometric method. The total of 23 cases was subject to this study. The respiratory organs (trachea, lungs) were taken from autopsies of newborn babies exited from different causes. For this purpose the tissues were fixed in formalin (10%) solution, cut serially in 7micro and 10micro slabs. Volume density of the respiratory system was assessed stereometricaly using Universal testing system Weibel M 42. We observed that volume density of epithelia, musculature and glands were proportionally present in the tracheal tissue. Cellular interstitial tissue is consistently increasing and corresponds to the developmental stages of the newborn babies. The density of tracheal ganglions is greater in premature ages of immature and premature newborns (p<0,05). Decreased number of ganglion cells is observed in mature ages (p<0,05). This is caused by intensive ramification of ganglions from serosa to deeper layers of trachea right to epithelium. Medium diameter of tracheal ganglions is greater in mature newborn babies and corresponds to developmental ages of babies.

PubMed Disclaimer

Figures

FIGURE 1
FIGURE 1
M42 test-system.
FIGURE 2
FIGURE 2
The observed testing point phase of tracheal ganglions with testing system Μ 42, the histoenzymatic staining in fresh tissues (indoxyl method for esterase 400 x) (3).
FIGURE 3
FIGURE 3
Tracheal volume density in the analyzed fetuses (X±SEM)
FIGURE 4
FIGURE 4
Numeric density of tracheal ganglions in the analyzed fetuses (X±SEM)
FIGURE 5
FIGURE 5
Distributions of ganglion cells in the structure of tracheal tissue in live borne babies deceased after birth and fetuses exited in different weeks of gestations:

  1. Cholinereactivity well expressed in perichondrial mature ganglion containing 21 ganglion cells in mature babies (Indoxyl method for esterase; 650x).

  2. Ganglion cells in lamina propria of tracheal tissue with expressed choline reactivity, with dendritic and axonal extensions around tracheal glands in prematurely born babies (Indoxyl method for esterase; 400 x).

  3. Choline reactivity in smooth musculature tracheal ganglion in prematurely born babies, which contains 4 cells with oval appearance (Indoxyl method for esterase; 400 x).

  4. Tracheal ganglion cells arranged in the crown form with expressed choline reactivity in mature born babies (Indoxyl method for esterase; 1600 x).

  5. Ganglion cells in lamina propria of tracheal tissue with axonal extension (Indoxyl method for esterase; 400 x).

  6. Group of ganglion cells with perivascular arrangement in tracheal tissue in prematurely born babies (Indoxyl method for esterase; 650x).

See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Baluk P, Fujiwara T, Matsuda S. The fine structure of the ganglia of the guinea-pig trachea. Cell Tissue Res. 1985;239:51–60. - PubMed
    1. John W.M. Neurogenesis of patterns for neurogenesis of gasping:noeud vital or noeuds vitals. J. Appl. Physiol. 1996;81:1865–1877. - PubMed
    1. Shabani R, Hundozi Z, Islami H. Evoluimi i dendësisë numerike neuronale në bëthamat dorsale motorike dhe solitare të nervit vagus në palcën e zgjatur te të porsalindurit. Praxix medica. 2002;44:10–17.
    1. Kanemoto Y, Ishibashi H, Doi A, Akaike N, Ito Y. An electro-physiological study of muscarinic and nicotinic receptors of rat paratracheal ganglion neurons and their inhibition by Z-338. Br. J. Pharmacol. 2002;135:1403–1414. - PMC - PubMed
    1. Shibata K, Taketani K. Excitatory effect of noradrenaline on rat airway parasympathetic ganglion neurons. Fukuoka Igaku Zasshi, 2001;92:377–383. - PubMed

Substances