Age susceptibility of red deer (Cervus elaphus) to paratuberculosis

Abstract

The aim of this study was to measure the relative susceptibility of three age classes of red deer (Cervus elaphus) to the various manifestations of infection and disease states due to Mycobacterium avium subsp. paratuberculosis (MAP) after experimental oral challenge. Three groups of seronegative female deer (30 three-month-old weaners, 20 fifteen-month-old yearlings and 20 adults) received four oral doses of approximately 10(9) colony forming units (cfu) of a bovine strain of MAP. They were monitored for 50 weeks by weighing, blood sampling for immunological assays, skin testing and faecal culturing. Clinically affected animals were promptly euthanised and the remaining deer were killed at the end of the study. Necropsies were carried out and samples of intestine and associated lymph nodes were taken for culture and histopathology from all deer. Ten weaners developed clinical paratuberculosis and were euthanased 20-28 weeks post-challenge (pc). No clinical cases occurred in the yearlings or adults. All 10 clinically affected weaners had severe gross and histopathological lesions typical of paratuberculosis (Johne's disease). At slaughter, gross lesions were seen in the jejunal lymph nodes of 8/17 weaners, 2/19 yearlings, and 0/20 adults. MAP was cultured from samples of the intestine and/or lymph nodes from all 10 clinical cases and from 16/17 weaners, 19/19 yearlings and 18/20 adult hinds at slaughter. Lesion Severity Scores of deer slaughtered 50 weeks pc averaged 4.9, 3.5 and 1.1 for the weaner, yearling and adult groups, respectively. At some time over the course of the trial, 24/28 weaners were antibody positive and immediately prior to slaughter, 13/17 weaners, 15/19 yearlings and 3/20 hinds were antibody positive. There is a strong age-related resistance against clinical disease and subclinical disease, but not to infection with MAP, after heavy oral challenge.